Insertion junction: CLIP2.075946_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.075946
Chromosome:chromosome 3
Location:1266758
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g149900 (1 of 1) IPR027369 - DinB/YfiT-like putative metal-dependent hydrolase 3'UTR
Cre03.g149950 (1 of 83) IPR029044 - Nucleotide-diphospho-sugar transferases 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGTTTGTACTGATCAAGGCCATCACGCACATAACATAGCAGGTCAGGTT
Internal bar code:TATCTTGCATTATTGTCACGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1158
LEAP-Seq percent confirming:38.4615
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:CTCTCTCTGCCTCTCCTGGT
Suggested primer 2:TAGGTGTGATACGGCCTGGA