| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.075951 |
| Chromosome: | chromosome 9 |
| Location: | 3258235 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g394917 | (1 of 4) PTHR16255//PTHR16255:SF4 - UNCHARACTERIZED // SPORULATION PROTEIN RMD8 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCAGCCCTCAAGGGCGCCCCAGTCAGCCCCAGTGAACGCCTGTGCATCC |
| Internal bar code: | TTGGGATGGTAGCACTAAGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 669 |
| LEAP-Seq percent confirming: | 7.69231 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACTGTGCATGCGTGTGTT |
| Suggested primer 2: | TTTTGGGAACTTGGGGGCTT |