Insertion junction: CLIP2.075952_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.075952
Chromosome:chromosome 12
Location:2680391
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g505250 FAP288 (1 of 3) PF13202//PF13499 - EF hand (EF-hand_5) // EF-hand domain pair (EF-hand_7); EF Hand Containing Flagellar Associated Protein 288 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGAAGTCACCACCTGGCCCCGGAATCTGCATTTCGAATCATTACTGATAT
Internal bar code:TTGTATTACTAATAAGCTGGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:5137
LEAP-Seq percent confirming:79.661
LEAP-Seq n confirming:47
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:59

Suggested primers for confirmation by PCR

Suggested primer 1:ATGTTCAACGTGGTTGCGTG
Suggested primer 2:CTAGTAGGGGCAGGAGTGGT