Insertion junction: CLIP2.075964_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.075964
Chromosome:chromosome 10
Location:5620664
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCCTCCCTCAGCACCACAGCACGCTCGCTACCTGTTAGCTGATAGCTGC
Internal bar code:CATTTGACTCTCATTAGAAACA

Confirmation - LEAP-Seq

LEAP-Seq distance:2375
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:TGAGTTCCGAGTTTGCCCTC
Suggested primer 2:GTCCCTGAAACAGCCGTGTA