| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.075967 |
| Chromosome: | chromosome 6 |
| Location: | 7276499 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g299100 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGTGCAAGTGGGGCTGTGGAGGATGATGGTGACCCGCAAGTCCAGGGT |
| Internal bar code: | CTGGGAGGGTCACTCGTTCGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5131 |
| LEAP-Seq percent confirming: | 93.3333 |
| LEAP-Seq n confirming: | 42 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTTGGGTGAACGTGCAAG |
| Suggested primer 2: | AATTGCTTGGCATTGCTGGG |