| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.075973 |
| Chromosome: | chromosome 13 |
| Location: | 2926559 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g583500 | TAF2,GEX2 | (1 of 5) IPR000104//IPR016024 - Antifreeze protein, type I // Armadillo-type fold | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGTTAAGGATTGGGGGGGGGGGGTTGCTGTATGTGGAGAACAGAGTGGT |
| Internal bar code: | TATCTGAGTTTACGGCGGATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2540 |
| LEAP-Seq percent confirming: | 67.8571 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGTGGAAACCGTAGTCACA |
| Suggested primer 2: | TCCTCCTCCTGCTCAGTACC |