| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.076008 |
| Chromosome: | chromosome 16 |
| Location: | 4406521 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g668300 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAACCGCCTGCGTGAGCGCGAACTTTGCGCTTGACGGCGTCTGCCCGCG |
| Internal bar code: | GGTTCGTGCCAGTGACTTTGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1342 |
| LEAP-Seq percent confirming: | 93.3333 |
| LEAP-Seq n confirming: | 56 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTATGAGTGTTCGCGCGAC |
| Suggested primer 2: | CGGGAGCTTCATGGAAACCT |