| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.076010 |
| Chromosome: | chromosome 9 |
| Location: | 2909450 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g390450 | POB15 | Proteome of basal body 15; (1 of 88) IPR011333 - POZ domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAATCAGCGCCTCCATCTGCCGCTGGGCTCACCAGTCGTCCCACCCTGC |
| Internal bar code: | TGCGGTCCATACCGTTTATGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1461 |
| LEAP-Seq percent confirming: | 85.5422 |
| LEAP-Seq n confirming: | 71 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 83 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGCGGTACTGATGTCGTG |
| Suggested primer 2: | AGGGAGCAAACACACACACA |