| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.076017 |
| Chromosome: | chromosome 4 |
| Location: | 3699590 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g229100 | (1 of 2) 2.7.1.158 - Inositol-pentakisphosphate 2-kinase / IP5 2-kinase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACAGTCAACTTAACAAGACCTGACCCGGTACAACAAGCAAACGCCCCTC |
| Internal bar code: | TATTGCGCCGTTACACTTCTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 561 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAAGTGCACCCATCCATGC |
| Suggested primer 2: | GCATCGATCTGCAGCATGTG |