Insertion junction: CLIP2.076023_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.076023
Chromosome:chromosome 11
Location:1153926
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g467678 COP6,HKR2 (1 of 5) IPR001660//IPR013761 - Sterile alpha motif domain // Sterile alpha motif/pointed domain; Histidine cyclase rhodopsin 2 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACATTTGTATGCATGAGCAATGGGGCCACACCACTTTTGCCAATCGCCT
Internal bar code:CTCGGAAGGTTCGCTAGATGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:2191
LEAP-Seq percent confirming:65.0
LEAP-Seq n confirming:91
LEAP-Seq n nonconfirming:49
LEAP-Seq n unique pos:140

Suggested primers for confirmation by PCR

Suggested primer 1:CAGAACGCTCCACAACCTCT
Suggested primer 2:GCGTGCTATGTAGGTTTGCG