| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.076024 |
| Chromosome: | chromosome 1 |
| Location: | 1956127 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g010816 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCGAACTCAGGGAGGGCCACAACAGATCATCCGTCCCACACGGGCACG |
| Internal bar code: | CTAGAGTGAGCCCGGGTGATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 845 |
| LEAP-Seq percent confirming: | 76.6667 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTGTCTTTGCGCTTTGCT |
| Suggested primer 2: | ACTCGAGCATTATAGGCGGC |