| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.076030 |
| Chromosome: | chromosome 14 |
| Location: | 377952 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g610300 | (1 of 88) 3.6.4.12 - DNA helicase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGGAATAGTCAATAAGAATGCCGCGCGGATAAAGTCTTGATTTGGCTCT |
| Internal bar code: | GAGATAGTTCACTGGTTGTATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 897 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAGAGGGGAGAGGCATGAG |
| Suggested primer 2: | GCTCTTGGTGCTTCTGTCCT |