Insertion junction: CLIP2.076036_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.076036
Chromosome:chromosome 14
Location:3184053
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g628702 (1 of 1) PF11919 - Domain of unknown function (DUF3437) (DUF3437) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACGGTGTAACCGGACACGTGCGCTGACCCGTGCTGTACTGTAGGGGGA
Internal bar code:TGATATTGCAAGCAAGTTTGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:670
LEAP-Seq percent confirming:42.1053
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:AAATGATGGACTGGCTGGGG
Suggested primer 2:AAGTCATGTCAGCAGTGCGA