| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.076050 |
| Chromosome: | chromosome 10 |
| Location: | 2211196 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g433650 | TMC3 | (1 of 4) 2.1.1.178 - 16S rRNA (cytosine(1407)-C(5))-methyltransferase; putative tRNA (cytosine-5)-methyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTACATCCTCAGGACGTCTGTAGCAGCCGCAAGCCGCTCGCACCCCCAG |
| Internal bar code: | TGTTTGTTAATTCCTGAGAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2783 |
| LEAP-Seq percent confirming: | 41.8182 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGAGCTTCAAGGGACTGG |
| Suggested primer 2: | CTGACTTCTGGGGCTTGGAG |