Insertion junction: CLIP2.076056_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.076056
Chromosome:chromosome 6
Location:952896
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g256420 (1 of 1) K15196 - transcription factor IIIB 90 kDa subunit (BRF1, GTF3B) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACACAGCCCCATGCTCCTCGCTGGCTCCACAGTTCCAGCAGTTCAGCAG
Internal bar code:GCTCTTGCAAACGCCCCATGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:665
LEAP-Seq percent confirming:90.9091
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:GTTCGCGGATGATTTGGAGC
Suggested primer 2:CCAGGGAGGGCAAGTTAGTG