Insertion junction: CLIP2.076059_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.076059
Chromosome:chromosome 17
Location:324327
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g698150 CYCR4 Cyclin-related protein; (1 of 10) PTHR10177 - CYCLINE 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCAAAACTATGACCACCTCACCTCCCAGGCGCGTGCGTCAGCGGACAGC
Internal bar code:CGCTAGCTAATACATGTAACGG

Confirmation - LEAP-Seq

LEAP-Seq distance:2115
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:19
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:ATTGCGTGTGTGTGTTTCGG
Suggested primer 2:CAGGACCACACCACTGCTAG