| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.076074 |
| Chromosome: | chromosome 2 |
| Location: | 4684504 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g107900 | (1 of 173) IPR029058 - Alpha/Beta hydrolase fold | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATATGAGGGTAGACGTTGCGGCTCTGCGACGCGTGAGCAGGCAGCGGCGT |
| Internal bar code: | ATGTACTAATTTACAACACTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3377 |
| LEAP-Seq percent confirming: | 86.6667 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGCGTGTCTTCGATATGGA |
| Suggested primer 2: | AGCGCGTTAGTATACCGGTG |