| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.076085 |
| Chromosome: | chromosome 8 |
| Location: | 3807747 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g381300 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTCGATAAGCTCAGGTGCTCGTAGCTAAGCAGTGGGCGTAAAAGCTGTT |
| Internal bar code: | GTCTTTCCCTCCGGGACTGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4294 |
| LEAP-Seq percent confirming: | 59.0909 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGAACCCTGTCCCATCAC |
| Suggested primer 2: | GATAGATGGGGGCGGACAAG |