| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.076087 |
| Chromosome: | chromosome 11 |
| Location: | 2178657 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467735 | (1 of 752) IPR027417 - P-loop containing nucleoside triphosphate hydrolase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTGGTCAGGCTGTGTGAAGATACCCGCAGATCAGCCAGTGCTCGTATCT |
| Internal bar code: | AAAGTTTGAGGGGTTGGGAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2674 |
| LEAP-Seq percent confirming: | 98.5507 |
| LEAP-Seq n confirming: | 68 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 69 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCAAAATATTCCGCCGCA |
| Suggested primer 2: | TACACAAGCCTGCTGACCTG |