| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.076103 |
| Chromosome: | chromosome 6 |
| Location: | 1241096 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g258600 | (1 of 2) K01061 - carboxymethylenebutenolidase (E3.1.1.45) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGCGCCGCACACCATCATCGGAGGGTCATCCGCAGGACGTTCCCACTT |
| Internal bar code: | GGTTCCAAGATAGGTAGTTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2382 |
| LEAP-Seq percent confirming: | 97.5 |
| LEAP-Seq n confirming: | 39 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGCAAGTTTGAACACCGG |
| Suggested primer 2: | GCATGGAAAGGCATGAGCAG |