| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.076111 |
| Chromosome: | chromosome 15 |
| Location: | 5564684 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre46.g761047 | (1 of 3) IPR000104//IPR027417 - Antifreeze protein, type I // P-loop containing nucleoside triphosphate hydrolase | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCACCATGGGCTGGCGGGGGAAAAACCCCCGCTCCCCACTCCATGGTG |
| Internal bar code: | GCAGCTTGTGACAAGCAGTCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1698 |
| LEAP-Seq percent confirming: | 59.6154 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGAGAGCGTGTGGACACT |
| Suggested primer 2: | CGAAGGAACATGTTAGCGCG |