Insertion junction: CLIP2.076113_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.076113
Chromosome:chromosome 17
Location:2669288
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre17.g717000 (1 of 7) PF02190 - ATP-dependent protease La (LON) substrate-binding domain (LON_substr_bdg) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCCACTGCCAACCCCGCCCCACCCACCGCCCACAGGCTGAACCGCTCG
Internal bar code:ATGGATTTTCTTTCTTTTCTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:75
LEAP-Seq percent confirming:20.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:TCGATCACATGCTTCCCCAC
Suggested primer 2:ATCTCTCCCCACCTCCTCAC