Insertion junction: CLIP2.076119_3

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.076119
Chromosome:chromosome 3
Location:6424779
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g192950 (1 of 4) 2.7.11.13 - Protein kinase C / PKC 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTAGCCGAGTTTACGCGAAACCTTCTTCCTCGTTCCAGCGCTTTCCTTGG
Internal bar code:CTGCTGCTTAGCAGCTTCAAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:493
LEAP-Seq percent confirming:1.5873
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:62
LEAP-Seq n unique pos:63

Suggested primers for confirmation by PCR

Suggested primer 1:TACATCTCCGGCGCTGATTC
Suggested primer 2:AAGCTTCGGACGGTTGGATT