| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.076126 |
| Chromosome: | plastome |
| Location: | 75762 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| CreCp.g802294 | ChreCp029,psbH,2717030 | photosystem II reaction center protein H; (1 of 1) K02709 - photosystem II PsbH protein (psbH) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTTAAGGCTAGCTGCTAAGTCTTCTTTTCGCTAAGGTAAACTAAGCAA |
| Internal bar code: | TGTGAGATTGTGGGCCTTTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 463 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACGATGGGGGCTTTTTGT |
| Suggested primer 2: | GGGGAAAAGGGACTGCCTAC |