| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.076129 |
| Chromosome: | chromosome 5 |
| Location: | 1850775 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g235600 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTCACTGCGACTCCCCAACCCGCCTCGTCGTGTCGCCGAGTTTGAGAG |
| Internal bar code: | AATATCGGTTCTTATAATCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3609 |
| LEAP-Seq percent confirming: | 2.1978 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 89 |
| LEAP-Seq n unique pos: | 91 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCATGCAAGTAATCCGGC |
| Suggested primer 2: | GATTGCGACGCTGATGCATT |