Insertion junction: CLIP2.076151_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.076151
Chromosome:chromosome 9
Location:3166524
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g394399 (1 of 1) K14767 - U3 small nucleolar RNA-associated protein 3 (UTP3, SAS10) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGAAATGTCTTTCAGATGCGGATTGCTGTTACGCCCCCGCGGCAAACGT
Internal bar code:CCGGCCACGAGTTGTATAGCCT

Confirmation - LEAP-Seq

LEAP-Seq distance:1494
LEAP-Seq percent confirming:96.2963
LEAP-Seq n confirming:26
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:GTATTGGTCGTGAATGGCGC
Suggested primer 2:GCTGAGGTCTCTGCGAGTTT