| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.076172 |
| Chromosome: | chromosome 16 |
| Location: | 5867734 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g679550 | FAP277 | Flagellar Associated Protein 277; (1 of 1) K13963 - serpin B (SERPINB) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGTCGAACGACTCACCCGCGCCGTTGAGGGCCAGCGTGAGTGCGTAAA |
| Internal bar code: | TTTATTACAGGACGAAGGTTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 734 |
| LEAP-Seq percent confirming: | 20.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAATAGTCAAAGGGGCCCGG |
| Suggested primer 2: | GCACGGTGTTTTGGCAGATT |