| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.076175 |
| Chromosome: | chromosome 10 |
| Location: | 1718888 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g430050 | UBC20 | E2 Ubiquitin conjugating enzyme; (1 of 1) K10583 - ubiquitin-conjugating enzyme E2 S (UBE2S, E2EPF) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTCAACACAGGAAAAACTTATACTGACACATACAGTATTTGTAACGTTG |
| Internal bar code: | TCATGGTGGGAGTAAAATTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5573 |
| LEAP-Seq percent confirming: | 97.561 |
| LEAP-Seq n confirming: | 40 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGAGGGTTGTACGTGGTCG |
| Suggested primer 2: | TAACGTTTGAGCGACGGTCA |