Insertion junction: CLIP2.076188_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.076188
Chromosome:plastome
Location:29041
Confidence (%):63
Locus systematic id Locus common name Defline Feature
CreCp.g802278 2717059,rpl5,ChreCp016 50S ribosomal protein L5; (1 of 2) PF00673 - ribosomal L5P family C-terminus (Ribosomal_L5_C) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GATTCCACAATTTTTTGGTTTTGTGATGCATCACCAATACCACGGTTAAT
Internal bar code:TCGGAATTATCGGATACTTGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:181
LEAP-Seq percent confirming:75.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CCCCTTCGGGCAAGTAAACT
Suggested primer 2:GCCACTGACGTCCACTACAA