| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.076192 |
| Chromosome: | chromosome 10 |
| Location: | 6393388 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g464325 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGCTTTTAAGACGCCGCCTCCTGTCATGCCACCGGGGAGCTCTTCGTG |
| Internal bar code: | CTCCATTCCCATGGGACATTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2075 |
| LEAP-Seq percent confirming: | 4.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCGAGGTGTTGTTTCCGAG |
| Suggested primer 2: | GACTGGGCAGGCTTTCTGAT |