| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.076221 |
| Chromosome: | chromosome 1 |
| Location: | 6812191 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g048850 | (1 of 1) PF01342//PF01388 - SAND domain (SAND) // ARID/BRIGHT DNA binding domain (ARID) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGTGGACGTAATCCATGTGGGCAGTGTTGACGTATCACCCTCCGTCAC |
| Internal bar code: | AAGGTGGAAATAATGCCGACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2249 |
| LEAP-Seq percent confirming: | 98.0392 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTGATTCATGCGCGTTAA |
| Suggested primer 2: | ATGAGCAGCTATCGGTCGTG |