Insertion junction: CLIP2.076222_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.076222
Chromosome:chromosome 16
Location:5955106
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g678900 HIK,HKR6,HIK6 (1 of 3) IPR001789//IPR011006 - Signal transduction response regulator, receiver domain // CheY-like superfamily; Response regulator of two-component system 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGACAAATTCATCTTCTCATCATTTCATGGCGACTGCGCACCGCCTTCT
Internal bar code:TGATCTGAACCCGGAGCAAAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:7235
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:117
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:117

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGCAGTACGGTTTTTGCA
Suggested primer 2:GGAGTTCGGACAGTGGACAG