Insertion junction: CLIP2.076223_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.076223
Chromosome:chromosome 2
Location:4739136
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g108400 DAD1 Putative defender against death (DAD) protein; (1 of 1) K12668 - oligosaccharyltransferase complex subunit epsilon (OST2, DAD1) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCACCTCCGCACCTTCTTACACCCAGGCACCTGTCATCCACTCCTATAGA
Internal bar code:GTCAGGGTCTATTTCATGCTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1948
LEAP-Seq percent confirming:96.2963
LEAP-Seq n confirming:26
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:AGCCAACAAGGAGTTCTCGG
Suggested primer 2:AGTTCACTCAGCTGCCGTAC