Insertion junction: CLIP2.076226_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.076226
Chromosome:chromosome 9
Location:4536844
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g401108 (1 of 274) IPR020683 - Ankyrin repeat-containing domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGGCCGGGCCGGGGTCAGGACTTGGCAGCGGGGACCTTTCTTATCTATG
Internal bar code:GTGAGCGGGGGGGACGGCACCG

Confirmation - LEAP-Seq

LEAP-Seq distance:1463
LEAP-Seq percent confirming:61.5385
LEAP-Seq n confirming:40
LEAP-Seq n nonconfirming:25
LEAP-Seq n unique pos:65

Suggested primers for confirmation by PCR

Suggested primer 1:ATTGCGCATCACCGTATTGC
Suggested primer 2:AACCTCGTTTCGGTGGAGTC