| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.076230 |
| Chromosome: | chromosome 9 |
| Location: | 167796 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g386734 | (1 of 2) PTHR31646//PTHR31646:SF1 - FAMILY NOT NAMED // ALPHA-1,2-MANNOSYLTRANSFERASE MNN2 | 3'UTR | |
| Cre09.g386735 | DLA1 | Dihydrolipoamide acetyltransferase; (1 of 1) PTHR23151//PTHR23151:SF9 - DIHYDROLIPOAMIDE ACETYL/SUCCINYL-TRANSFERASE-RELATED // DIHYDROLIPOYLLYSINE-RESIDUE ACETYLTRANSFERASE COMPONENT OF PYRUVATE DEHYDROGENASE COMPLEX, MITOCHONDRIAL | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATGCGCCTCTGCTTTAGAACCGCAGCCATTTTGTAACAGTACTTCGTTA |
| Internal bar code: | TTTCAGGATTTGTGTGGACGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3942 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 65 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 65 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCGATCATTGGTGAAGTC |
| Suggested primer 2: | GCTGAACCCCCATGTCGTAA |