Insertion junction: CLIP2.076238_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.076238
Chromosome:chromosome 13
Location:456309
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g564900 MRP3 (1 of 18) 3.6.3.44 - Xenobiotic-transporting ATPase / Steroid-transporting ATPase; ABC transporter, multidrug resistance associated protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTTCAAGAGCGACAGGTTTGAGGAGGGCAGATACTGCGAGTGTCGGCGC
Internal bar code:CTACTAAAGCTACGTCCGTAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:3592
LEAP-Seq percent confirming:75.4098
LEAP-Seq n confirming:92
LEAP-Seq n nonconfirming:30
LEAP-Seq n unique pos:122

Suggested primers for confirmation by PCR

Suggested primer 1:TATGCGTATGTCAGGCGGTC
Suggested primer 2:CGATCTTTGTACAGCGTGCG