Insertion junction: CLIP2.076238_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.076238
Chromosome:chromosome 13
Location:456311
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g564900 MRP3 (1 of 18) 3.6.3.44 - Xenobiotic-transporting ATPase / Steroid-transporting ATPase; ABC transporter, multidrug resistance associated protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCCATCCACACCAGGAAACCACAGAATATGCCAACCCTAGTGACCCCCA
Internal bar code:GGTTTGTGGCGTTGGGTGTCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:6301
LEAP-Seq percent confirming:93.5484
LEAP-Seq n confirming:29
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:31

Suggested primers for confirmation by PCR

Suggested primer 1:CGATCTTTGTACAGCGTGCG
Suggested primer 2:TATGCGTATGTCAGGCGGTC