| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.076240 |
| Chromosome: | chromosome 3 |
| Location: | 4894193 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g178650 | MCM6 | (1 of 1) K02542 - DNA replication licensing factor MCM6 (MCM6); Minichromosome maintenance protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTCATCACACGCACTACAGCCAACCCTCAACCTGCGCCCCAGGATGCT |
| Internal bar code: | CGAATAAATTCGTTCGTGCAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4845 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 41 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAGAGCTCCGCCTAACGTG |
| Suggested primer 2: | AGAATAGCGTACGGTCAGCG |