| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.076244 |
| Chromosome: | chromosome 13 |
| Location: | 2694205 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g581550 | THG1 | Histidine tRNA 5'-guanylyltransferase; (1 of 1) 2.7.7.79 - tRNA(His) guanylyltransferase / Histidine tRNA guanylyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCAGTGCACAGGCGCGGGTTCCGTGGGTGGCTGTTGGCGCCAGGCAGC |
| Internal bar code: | AATGCAAACTAGGTAGCGTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2667 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACAGGGAGGTCAGGTAGGC |
| Suggested primer 2: | GCATCTGCTTCTATCCCGCT |