| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.076250 |
| Chromosome: | chromosome 3 |
| Location: | 8921814 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g204801 | (1 of 1) K10606 - E3 ubiquitin-protein ligase FANCL (FANCL, PHF9) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCTCGCGGCCGCAGCCGTCGACCAGCCGGAGACTCAGCCGGGCGGCGT |
| Internal bar code: | TGAAAGCCTTACGTCTTGGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 205 |
| LEAP-Seq percent confirming: | 56.5217 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGGTACCATGCTCGTTGCG |
| Suggested primer 2: | GCATTGCGGTCGATGTCTTC |