| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.076255 |
| Chromosome: | chromosome 16 |
| Location: | 6513551 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g674600 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGCGGTGAGCCGGGCCTGGGGTTCCTCCCCTCTCCGGAGGCGAGGAGC |
| Internal bar code: | TGCCATTTTGATCCGCTCTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 791 |
| LEAP-Seq percent confirming: | 55.5556 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCAGTGTGCGTACAAGGAG |
| Suggested primer 2: | TGGACATCACACGGAACCAG |