| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.076264 |
| Chromosome: | chromosome 4 |
| Location: | 394203 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217913 | MCP19 | Mitochondrial substrate carrier protein; (1 of 1) PTHR24089:SF133 - PROTEIN Y37B11A.3 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAACACCTTCCCCCCCCGTCGTTACATCGACTACTGTGTACCTTTGCACC |
| Internal bar code: | ATGAGCAATGAGCAGCGGTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 75 |
| LEAP-Seq percent confirming: | 7.69231 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAACACCGTGCGAAGGTAT |
| Suggested primer 2: | ACGTGAGAGCACAAGGACAG |