| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.076280 |
| Chromosome: | chromosome 3 |
| Location: | 5015140 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g179820 | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAAGCTTGCTATTTCATCTTGCATGCGTTTAATATCGTCGCTGCCGCC |
| Internal bar code: | TAATGCGCGCGCGGATGAGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 114 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGTTGCCACCTCCCCTAC |
| Suggested primer 2: | CGTGTGAACTCAGAGGGCAT |