| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.076307 |
| Chromosome: | chromosome 12 |
| Location: | 3533710 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g496500 | PHC70 | Putative pherophorin-chlamydomonas homolog; (1 of 71) PF12499 - Pherophorin (DUF3707) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGTGGTTGGAGGTGTGGAACTGTGCATGGCTTGGAGTAAGGTGGCAAG |
| Internal bar code: | TACAGTTGGTTTTTGTAGCTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 242 |
| LEAP-Seq percent confirming: | 9.67742 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTGTTGTAAGCGAGCCAT |
| Suggested primer 2: | ATTGCGGTTAGGACACCTGG |