Insertion junction: CLIP2.076309_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.076309
Chromosome:plastome
Location:20195
Confidence (%):63
Locus systematic id Locus common name Defline Feature
CreCp.g802271 ChreCp009,2717052,chlL light-independent protochlorophyllide reductase iron-sulfer ATP-binding protein; (1 of 1) K04037 - light-independent protochlorophyllide reductase subunit L (chlL) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAAGATTATCATTATGAAGATATTTGGCCCGAAGATGTTATTTACGGAGG
Internal bar code:CAATCGATATTGCTTCGCTGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:103
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CCCGCTAAACGCAATGGATG
Suggested primer 2:CCTCCTTCCCCTTCCCCTT