| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.076312 |
| Chromosome: | chromosome 16 |
| Location: | 4766652 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g687850 | PTK22 | (1 of 24) IPR006553 - Leucine-rich repeat, cysteine-containing subtype; Protein of unknown function | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGACAAAGTGAGGCCGGTTGGGGTACGGTGCCATGAGCCCACCACGAAG |
| Internal bar code: | CTTGGGTCCTACACATGATCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 478 |
| LEAP-Seq percent confirming: | 42.8571 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGTCGTCAATTGTACGGCA |
| Suggested primer 2: | CGAGACATACGGAGTCGGTG |