| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.076315 |
| Chromosome: | chromosome 6 |
| Location: | 1156670 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g258000 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCAAGGGCCGGACCAGATTTCTTCGCAGATTGGCCCAACCACAGGTGC |
| Internal bar code: | TTGCAATCGGAATTTCTTTTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 165 |
| LEAP-Seq percent confirming: | 2.27273 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCCCCTGGGAAAAGGTTT |
| Suggested primer 2: | AAGAACGCACTTATGGCCCA |