| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.076350 |
| Chromosome: | chromosome 11 |
| Location: | 1775184 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467900 | (1 of 10) PF13578 - Methyltransferase domain (Methyltransf_24) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGAGGACCCCACCGCCACGTTGTGTGAGCGTGCCGGAGTGCGCGTTCG |
| Internal bar code: | GTGCGGCGCCTTAGCGATCGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1506 |
| LEAP-Seq percent confirming: | 64.2857 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTGCCAGCAATCCCAAAA |
| Suggested primer 2: | CGGGTAGACGGTGTATGACG |