Insertion junction: CLIP2.076352_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.076352
Chromosome:chromosome 3
Location:7901275
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g202500 (1 of 1) PF04836//PF05004 - Interferon-related protein conserved region (IFRD_C) // Interferon-related developmental regulator (IFRD) (IFRD) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGAATGAACGAGGAAGACTCGCCATCTGCAAGCACGTGGATGCGGAGGT
Internal bar code:AGTTTAGAATCTTTGTAGGTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:2419
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:29
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:AGCTTCAATCGCCCACATCA
Suggested primer 2:GCGCTGGGCAGTATCATAGT