| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.076361 |
| Chromosome: | chromosome 1 |
| Location: | 4409973 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g030050 | PRPL34 | Chloroplast ribosomal protein L34; (1 of 1) K02914 - large subunit ribosomal protein L34 (RP-L34, MRPL34, rpmH) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACTCCAATGCGCGAGCGCGACACACCATGCCTAAGGTTGACAGCCTTCG |
| Internal bar code: | GGCTGAATCTTGGCTCGTTCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 287 |
| LEAP-Seq percent confirming: | 20.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACATGCTTCCGTCAGCTT |
| Suggested primer 2: | CTACGCTTGTACTGCCCACA |